Enzimas de hongos de pudrición blanca involucradas en la degradación de lignina / Enzymes of white rot fungi involved in lignin degradation

ABSTRACT

The presence of oxidases and peroxidases was tested qualitatively in 12 strains of white rot Basidiomycetes. Plate tests with gallic acid, tannic acid, guayacol, Poly R-478 and Azure B were used. Fomes sclerodermeus, Phlebia sp. and Pycnoporus sanguineus were selected for further studies because they produced the largest areas of degradation in all media tested. Poly R-478 degradation and manganese peroxidase, lignin peroxidase and laccase activities were measured in glucose-asparagine (N-sufficient) and Kirk (N-limited) media. The highest activities were produced by F. sclerodermeus cultured in glucose asparagine medium.