Respuesta humoral específica en ratones Balb/c inoculados con una librería genómica de expresión de Trypanosoma cruzi / Specific humoral response in Balb/c mice inoculated with a genome library of expression of Trypanosoma cruzi
Rev. cuba. med. trop
; 51(1): 20-25, ene.-abr. 1999.
Article
de Es
| LILACS
| ID: lil-333545
Bibliothèque responsable:
BR1.1
RESUMO
A genomic expression library of Trypanosoma cruzi (T. cruzi) was made using plasmid pcDNA3 as a vector, with which male mice from the Balb/c isogenic line were intramuscullary inoculated. It was used a positive control group that was administered soluble antigens of T. cruzi. Other 2 groups received genomic and plasmid DNA, respectively. One group was not immunized. Weekly blood samples were obtained from all the animals until the fourth week and 2 weeks after reimmunization to study the response of specific antibodies against the microorganism antigens by an indirect immunoenzymatic assay (ELISA). It was observed a significant increase of specific antibodies in the animals reimmunized with 50 micrograms of the library, as well as in the group immunized with soluble antigens of T. cruzi.
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Trypanosoma cruzi
/
Anticorps antiprotozoaires
/
Banque génomique
/
ADN des protozoaires
Limites du sujet:
Animals
langue:
Es
Texte intégral:
Rev. cuba. med. trop
Thème du journal:
MEDICINA TROPICAL
Année:
1999
Type:
Article