Human mixed infections of Leishmania spp. and Leishmania-Trypanosoma cruzi in a sub Andean Bolivian area: identification by polymerase chain reaction/hybridization and isoenzyme
Mem. Inst. Oswaldo Cruz
;
98(2): 255-264, Mar. 15, 2003. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-334264
ABSTRACT
Parasites belonging to Leishmania braziliensis, Leishmania donovani, Leishmania mexicana complexes and Trypanosoma cruzi (clones 20 and 39) were searched in blood, lesions and strains collected from 28 patients with active cutaneous leishmaniasis and one patient with visceral leishmaniasis. PCR-hybridization with specific probes of Leishmania complexes (L. braziliensis, L. donovani and L. mexicana) and T. cruzi clones was applied to the different DNA samples. Over 29 patients, 8 (27.6 percent) presented a mixed infection Leishmania complex species, 17 (58.6 percent) a mixed infection Leishmania-T. cruzi, and 4 (13.8 percent) a multi Leishmania-T. cruzi infection. Several patients were infected by the two Bolivian major clones 20 and 39 of T. cruzi (44.8 percent). The L. braziliensis complex was more frequently detected in lesions than in blood and a reverse result was observed for L. mexicana complex. The polymerase chain reaction-hybridization design offers new arguments supporting the idea of an underestimated rate of visceral leishmanisis in Bolivia. Parasites were isolated by culture from the blood of two patients and lesions of 10 patients. The UPGMA (unweighted pair-group method with arithmetic averages) dendrogram computed from Jaccard's distances obtained from 11 isoenzyme loci data confirmed the presence of the three Leishmania complexes and undoubtedly identified human infections by L. (V.) braziliensis, L. (L.) chagasi and L. (L.) mexicana species. Additional evidence of parasite mixtures was visualized through mixed isoenzyme profiles, L. (V.) braziliensis-L. (L.) mexicana and Leishmania spp.-T. cruzi.The epidemiological profile in the studied area appeared more complex than currently known. This is the first report of parasitological evidence of Bolivian patients with trypanosomatidae multi infections and consequences on the diseases' control and patient treatments are discussed
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Trypanosoma cruzi
/
Leishmaniose cutanée
/
Maladie de Chagas
/
Leishmania
/
Leishmaniose viscérale
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
/
Humains
Pays comme sujet:
Amérique du Sud
/
Bolivie
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2003
Type:
Article
Pays d'affiliation:
Bolivie
/
France
Institution/Pays d'affiliation:
IRD Laboratoire d'Entomologie/BO
/
Institut de Recherche pour le Développement/FR
/
UMR/FR
/
Universidad Mayor San Andrès/BO
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