Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
Mem. Inst. Oswaldo Cruz
;
98(2): 269-271, Mar. 15, 2003. ilus, graf
Article
Dans Anglais
| LILACS
| ID: lil-334266
ABSTRACT
We describe a method for culturing over 90 percent pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Trypanosoma
/
Techniques de culture cellulaire
/
Macrophages
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2003
Type:
Article
Pays d'affiliation:
Colombie
Institution/Pays d'affiliation:
Universidad de Antioquia/CO
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