Microwave-enhanced of a method of silver impregnation applied in dermatohistopathology

RESUMO

An experimental development based on the combination of microwaves action with one of the methods of silver staining by Del RÝo Hortega is presented. Materials from normal and pathological tissues were studied. The stuff were fixed in 10 formalin and received a paraffined embedding. The impregnation was made with ammoniacal silver carbonate in a domestic microwave oven during twelve seconds at 375 w. As a result of this, a time shortage of the technique, the high degree of definition of the nuclear, cytoplasmatic and extracellular matrix (collagenus fibbers with an unsteady reticular representation) details and the easy visualization of mitosis was observed. Also, omitting the reduction, they were clearly evidenced the argirophilics granules (melanin pigment)