Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons
Braz. j. med. biol. res
;
36(7): 951-957, July 2003. ilus, graf
Article
Dans Anglais
| LILACS
| ID: lil-340691
RESUMO
A glutamate-sensitive inward current (Iglu) is described in rat cerebellar granule neurons and related to a glutamate transport mechanism. We examined the features of Iglu using the patch-clamp technique. In steady-state conditions the Iglu measured 8.14 ± 1.9 pA. Iglu was identified as a voltage-dependent inward current showing a strong rectification at positive potentials. L-Glutamate activated the inward current in a dose-dependent manner, with a half-maximal effect at about 18 æM and a maximum increase of 51.2 ± 4.4 percent. The inward current was blocked by the presence of dihydrokainate (0.5 mM), shown by others to readily block the GLT1 isoform. We thus speculate that Iglu could be attributed to the presence of a native glutamate transporter in cerebellar granule neurons
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Cervelet
/
Névroglie
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
2003
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Federal de Minas Gerais/BR
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