Total protein electrophoresis and RAPD fingerprinting analysis for the identification of aeromonas at the species level
Braz. j. microbiol
;
33(4): 358-362, Oct.-Dec. 2002. graf
Article
Dans Anglais
| LILACS
| ID: lil-342101
RESUMO
Fifteen well-defined strains of Aeromonas of thirteen species were analyzed by SDS protein electrophoretic analysis (SD-PAGE) and random amplified polymorphic DNA analysis (RAPD). The comparison between the patterns obtained by both methods allowed differentiating all the strains. Clusters formed by the unweighted pair group method with arithmetic averages applied to protein data correlates with the genetic and biochemical information about the species. The results show that protein fingerprinting has the potential to differentiate Aeromonas species, but the low qualitative variation indicates that this technique is not efficient of the characterization of strains within a species. Conversely, RAPD fingerprinting allows the identification of strains but the high variability limits its potential as an aiding method for species identification.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Protéines
/
Aeromonas
/
Électrophorèse
/
Cellules germinales
Type d'étude:
Etude diagnostique
/
Recherche qualitative
langue:
Anglais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2002
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade de Caxias do Sul/BR
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