Detection of rotavirus in dogs with diarrhea in Brazil
Braz. j. microbiol
;
34(1): 77-80, Jan.-Apr. 2003. ilus
Article
Dans Anglais
| LILACS
| ID: lil-344570
RESUMO
Rotavirus was detected by the enzyme-linked immunosorbent assay (ELISA) in the faeces of a diarrheic dog. Virus particles with morphology typical of rotavirus were visualized by direct electron microscopy. This sample was subsequently tested for the four main human serotypes (G1-G4), by ELISA with monoclonal antibodies. G genotyping was attempted by RT-PCR using G1-G6 and G8-G11 primers but no positive results could be yielded. Also using RT-PCR it was possible to characterize this canine strain as belonging to P[ 3] genotype. This is the first canine rotavirus detected in Brazil.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Techniques in vitro
/
Test ELISA
/
Rotavirus
/
Canine
/
Diarrhée
Type d'étude:
Etude diagnostique
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2003
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Fundação Nacional de Saúde/BR
/
Universidade de São Paulo/BR
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