Isolation of (GA)n microsatellite sequences and description of a predicted MADS-box sequence isolated from common bean (Phaseolus vulgaris L. )
Genet. mol. biol
;
26(3): 337-342, 2003. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-346325
ABSTRACT
The isolation of (GA)n microsatellites using a highly microsatellite-enriched library is described for the first time in common bean (Phaseolus vulgaris L.). A relatively simple and effective method to isolate DNA repeats from microsatellite-enriched libraries based on hybridization-capture of repeat regions using biotin-conjugated oligonucleotids and non-radioactive colony hybridization was carried out. PCR products from 200 to 800 bp were obtained and cloned. Of the 60 clones sequenced, 21 yielded (GA)n microsatellites with n values equal or higher than six. These (GA)n microsatellite-containing loci could be useful for further genetic mapping studies. A (GA)n microsatellite linked to a putative MADS-box gene was identified. This sequence, which represents the first MADS-box locus described to date in common bean, showed a very high similarity with other known MADS-box sequences and was grouped within the AGL2 subfamily cluster of the Arabidopsis MADS-box genes. The vicinity of microsatellites to some genes is also discussed
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Marqueurs génétiques
/
Répétitions microsatellites
/
Phaseolus
Type d'étude:
Étude pronostique
/
Facteurs de risque
langue:
Anglais
Texte intégral:
Genet. mol. biol
Thème du journal:
Génétique
Année:
2003
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Espagne
Institution/Pays d'affiliation:
Universidad de León/ES
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