Microsatellite polymorphism in wheat from Brazilian cultivars; inter- and intra-varietal studies
Genet. mol. biol
;
26(3): 349-353, 2003. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-346327
ABSTRACT
Eleven samples of wheat (Triticum aestivum) from different Brazilian cultivars and six American varieties were compared for polymorphism, using primers for nine different STR loci. STR analysis of DNA from single grains of the Brazilian varieties showed that for most loci there was very little intra-cultivar polymorphism. The polymorphic variation observed for Brazilian cultivars was similar to that seen in the American varieties. For the Brazilian cultivars PCR analysis could be performed on only one half of a grain. The American samples required more seeds for analysis. The nucleotide sequences of five amplified microsatellites selected at random from the Brazilian samples were also determined and compared to those of the Chinese Spring variety. Although generally the dinucleotide sequence repeat was preserved for most loci, there were significant differences in sequences interspersed within the repeat domain. This result suggested that it may be possible to unequivocally identify the geographical origin of the cultivar by inspection of the DNA sequences of the repeat region
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Triticum
/
Séquence nucléotidique
/
Réaction de polymérisation en chaîne
/
Répétitions microsatellites
/
Production végétale
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Genet. mol. biol
Thème du journal:
Génétique
Année:
2003
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Federal do Rio de Janeiro/BR
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