Your browser doesn't support javascript.
loading
Cross neutralizing antibodies in hamsters vaccinated with leptospiral bacterins produced with three serovars of serogroup Sejroe
Tabata, Rosana; Scanavini Neto, Hugo; Zuanaze, Marcelo Alexandre Fagnani; Oliveira, Eugenia Márcia de Deus; Dias, Ricardo Augusto; Morais, Zenaide Maria de; Ito, Fumio Honma; Vasconcellos, Silvio Arruda.
  • Tabata, Rosana; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal. São Paulo. BR
  • Scanavini Neto, Hugo; Laboratórios Biovet S/A. Vargem Grande Paulista. BR
  • Zuanaze, Marcelo Alexandre Fagnani; Laboratórios Biovet S/A. Vargem Grande Paulista. BR
  • Oliveira, Eugenia Márcia de Deus; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal. São Paulo. BR
  • Dias, Ricardo Augusto; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal. São Paulo. BR
  • Morais, Zenaide Maria de; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal. São Paulo. BR
  • Ito, Fumio Honma; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal. São Paulo. BR
  • Vasconcellos, Silvio Arruda; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal. São Paulo. BR
Braz. j. microbiol ; 33(3): 265-268, July-Sept. 2002. tab, graf
Article Dans Anglais | LILACS | ID: lil-349781
ABSTRACT
Three leptospiral bacterins, produced with different serovars of Serogroup Sejroe, namely the hardjo (bacterin A), wolffi (bacterin B) and guaricura (bacterin C), were evaluated in male hamsters (Mesocricetus auratus) by comparing the agglutinating and neutralizing antibodies titers using microscopic agglutination (MAT) and in vitro growth inhibition (GIT) tests. The immunization schedule was based on two 1.0 mL doses of non-diluted formalininactivated whole culture bacterin given through subcutaneous route with 10-day interval. The challenge was performed ten days after the second vaccine dose, when the animals were inoculated with 0.2 mL of non-inactivated cultures of each serovar through intraperitoneal route. On the 21st post-challenge day (PCD), all animals were bled and their sera were joined in pools (n=8) and tested by MAT and GIT. All vaccinated and control animals presented no clinical signs of leptospirosis after the challenge, but the serovar guaricura was isolated from the kidneys of control animals on the 21st PCD. The MAT results showed cross agglutinins between serovars hardjo and wolffi, and between wolffi and guaricura. The GIT results revealed the presence of cross neutralizing antibodies between serovars wolffi or guaricura against hardjo, wolffi and guaricura. It was found that the tested strain of serovar hardjo did not produce detectable levels of neutralizing antibodies, indicating its poor immunogenicity
Sujets)
Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Techniques in vitro / Cricetinae / Infections bactériennes à Gram négatif / Leptospira / Anticorps Type d'étude: Etude diagnostique Pays comme sujet: Amérique du Sud / Brésil langue: Anglais Texte intégral: Braz. j. microbiol Thème du journal: Microbiologie Année: 2002 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: A+BR / Universidade de São Paulo/BR

Documents relatifs à ce sujet

MEDLINE

...
LILACS

LIS

Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Techniques in vitro / Cricetinae / Infections bactériennes à Gram négatif / Leptospira / Anticorps Type d'étude: Etude diagnostique Pays comme sujet: Amérique du Sud / Brésil langue: Anglais Texte intégral: Braz. j. microbiol Thème du journal: Microbiologie Année: 2002 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: A+BR / Universidade de São Paulo/BR