Evaluation of an in-house specific immunoglobulin G (IgG) avidity ELISA for distinguishing recent primary from long-term human cytomegalovirus (HCMV) infection
Rev. Inst. Med. Trop. Säo Paulo
;
45(6): 323-326, Nov.-Dec. 2003. ilus
Article
Dans Anglais
| LILACS
| ID: lil-353983
ABSTRACT
This article describes the standardization and evaluation of an in-house specific IgG avidity ELISA for distinguishing recent primary from long-term human cytomegalovirus (HCMV) infection. The test was standardized with the commercial kit ETI-CYTOK G Plus (Sorin Biomedica, Italy) using 8 M urea in phosphate-buffered saline to dissociate low-avidity antibodies after the antigen-antibody interaction. The performance of the in-house assay was compared to that of the commercial automated VIDAS CMV IgG avidity test (bioMérieux, France). Forty-nine sera, 24 from patients with a recent primary HCMV infection and 25 from patients with a long-term HCMV infection and a sustained persistence of specific IgM antibodies, were tested. Similar results were obtained with the two avidity methods. All 24 sera from patients with recently acquired infection had avidity indices compatible with acute HCMV infection by the VIDAS method, whereas with the in-house method, one serum sample had an equivocal result. In the 25 sera from patients with long-term infection, identical results were obtained with the two methods, with only one serum sample having an incompatible value. These findings suggest that our in-house avidity test could be a potentially useful tool for the immunodiagnosis of HCMV infection.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Infections à cytomégalovirus
/
Cytomegalovirus
/
Anticorps antiviraux
/
Affinité des anticorps
Limites du sujet:
Femelle
/
Humains
langue:
Anglais
Texte intégral:
Rev. Inst. Med. Trop. Säo Paulo
Thème du journal:
Médecine tropicale
Année:
2003
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Estadual de Campinas/BR
/
Universidade de São Paulo/BR
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