Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stress
Braz. j. med. biol. res
;
37(2): 185-192, Feb. 2004. tab
Article
Dans Anglais
| LILACS
| ID: lil-354177
RESUMO
Exposure to stress induces a cluster of physiological and behavioral changes in an effort to maintain the homeostasis of the organism. Long-term exposure to stress, however, has detrimental effects on several cell functions such as the impairment of antioxidant defenses leading to oxidative damage. Oxidative stress is a central feature of many diseases. The lungs are particularly susceptible to lesions by free radicals and pulmonary antioxidant defenses are extensively distributed and include both enzymatic and non-enzymatic systems. The aim of the present study was to determine lipid peroxidation and total radical-trapping potential (TRAP) changes in lungs of rats submitted to different models of chronic stress. Adult male Wistar rats weighing 180-230 g were submitted to different stressors (variable stress, N = 7) or repeated restraint stress for 15 (N = 10) or 40 days (N = 6) and compared to control groups (N = 10 each). Lipid peroxidation levels were assessed by thiobarbituric acid reactive substances (TBARS), and TRAP was measured by the decrease in luminescence using the 2-2'-azo-bis(2-amidinopropane)-luminol system. Chronic variable stress induced a 51 percent increase in oxidative stress in lungs (control group 0.037 ± 0.002; variable stress 0.056 ± 0.007, P < 0.01). No difference in TBARS was observed after chronic restraint stress, but a significant 57 percent increase in TRAP was presented by the group repeatedly restrained for 15 days (control group 2.48 ± 0.42; stressed 3.65 ± 0.16, P < 0.05). We conclude that different stressors induce different effects on the oxidative status of the organism.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Stress physiologique
/
Peroxydation lipidique
/
Substances réactives à l'acide thiobarbiturique
/
Stress oxydatif
/
Poumon
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
2004
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Federal do Rio Grande do Sul/BR
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