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Leptospira spp detection by Polymerase Chain Reaction (PCR) in clinical samples of captive black-capped Capuchin monkey (Cebus apella)
Scarcelli, Eliana; Piatti, Rosa Maria; Fedullo, José Daniel Luzes; Simon, Faiproal; Cardoso, Maristela Vasconcellos; Castro, Vanessa; Miyashiro, Simone; Genovez, Margareth polide.
  • Scarcelli, Eliana; Instituto Biológico. Centro de Pesquisa e Desenvolvimento de Sanidade Animal. São Paulo. BR
  • Piatti, Rosa Maria; Instituto Biológico. Centro de Pesquisa e Desenvolvimento de Sanidade Animal. São Paulo. BR
  • Fedullo, José Daniel Luzes; Instituto Biológico. Centro de Pesquisa e Desenvolvimento de Sanidade Animal. São Paulo. BR
  • Simon, Faiproal; Instituto Biológico. Centro de Pesquisa e Desenvolvimento de Sanidade Animal. São Paulo. BR
  • Cardoso, Maristela Vasconcellos; Instituto Biológico. Centro de Pesquisa e Desenvolvimento de Sanidade Animal. São Paulo. BR
  • Castro, Vanessa; Instituto Biológico. Centro de Pesquisa e Desenvolvimento de Sanidade Animal. São Paulo. BR
  • Miyashiro, Simone; Instituto Biológico. Centro de Pesquisa e Desenvolvimento de Sanidade Animal. São Paulo. BR
  • Genovez, Margareth polide; Instituto Biológico. Centro de Pesquisa e Desenvolvimento de Sanidade Animal. São Paulo. BR
Braz. j. microbiol ; 34(2): 143-146, Apr.-Jun. 2003. ilus
Article Dans Anglais | LILACS | ID: lil-355163
RESUMO
Leptospirosis is a widely distributed zoonosis that affects domestic and wild animals, and that has the man as the end point of its epidemiological chain. Leptospirosis diagnosis in primates is more difficult than in other animal species, as clinical signs and lesions are less evident and antibody response is detected only for short periods. The aim of this article was to describe the detection of Leptospira spp using polymerase chain reaction (PCR), in clinical samples from one captive black-capped Capuchin monkey (Cebus apella), which presented characteristics compatible with leptospirosis (jaundice and haemorrhagic kdney) in the macroscopic post-mortem examination. A friable kidney fragment and urine sample were cultured and submitted to experimental inoculation in guinea pigs and PCR using genus specific primer pair targeting the 16S rRNA region from Leptospira interrogans serovar canicola. Isolation of the agent was negative both in culture and experimental inoculation. The PCR amplification of the clinical samples showed a 330 pb amplified fragment that corresponds to the Leptospira genus. Based on these results PCR was considered an important tool for leptospira detection in nonhumam primates, more sensitive and specific than other techniques, especially considering that the viability of the pathogen was not possible. These advantages enable the detection of the leptospiras in urine and kidney, even when autolysed, frozen or badly conserved, which prevented the isolation and experimental inoculation from positive results.
Sujets)
Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Réaction de polymérisation en chaîne / Leptospira / Leptospirose / Maladies des singes Type d'étude: Etude diagnostique Limites du sujet: Animaux langue: Anglais Texte intégral: Braz. j. microbiol Thème du journal: Microbiologie Année: 2003 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Instituto Biológico/BR

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Réaction de polymérisation en chaîne / Leptospira / Leptospirose / Maladies des singes Type d'étude: Etude diagnostique Limites du sujet: Animaux langue: Anglais Texte intégral: Braz. j. microbiol Thème du journal: Microbiologie Année: 2003 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Instituto Biológico/BR