Prediction of exposed domains of envelope glycoprotein in Indian HIV-1 isolates and experimental confirmation of their immunogenicity in humans
Braz. j. med. biol. res
;
37(5): 675-681, May 2004. tab
Article
Dans Anglais
| LILACS
| ID: lil-357538
ABSTRACT
We describe the impact of subtype differences on the seroreactivity of linear antigenic epitopes in envelope glycoprotein of HIV-1 isolates from different geographical locations. By computer analysis, we predicted potential antigenic sites of envelope glycoprotein (gp120 and gp4l) of this virus. For this purpose, after fetching sequences of proteins of interest from data banks, values of hydrophilicity, flexibility, accessibility, inverted hydrophobicity, and secondary structure were considered. We identified several potential antigenic epitopes in a B subtype strain of envelope glycoprotein of HIV-1 (IIIB). Solid- phase peptide synthesis methods of Merrifield and Fmoc chemistry were used for synthesizing peptides. These synthetic peptides corresponded mainly to the C2, V3 and CD4 binding sites of gp120 and some parts of the ectodomain of gp41. The reactivity of these peptides was tested by ELISA against different HIV-1-positive sera from different locations in India. For two of these predicted epitopes, the corresponding Indian consensus sequences (LAIERYLKQQLLGWG and DIIGDIRQAHCNISEDKWNET) (subtype C) were also synthesized and their reactivity was tested by ELISA. These peptides also distinguished HIV-1-positive sera of Indians with C subtype infections from sera from HIV-negative subjects.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Test ELISA
/
Protéine d'enveloppe gp41 du VIH
/
Protéine d'enveloppe gp120 du VIH
/
VIH-1 (Virus de l'Immunodéficience Humaine de type 1)
/
Immunogénétique
Type d'étude:
Étude pronostique
/
Facteurs de risque
Limites du sujet:
Humains
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
2004
Type:
Article
Pays d'affiliation:
Inde
Institution/Pays d'affiliation:
Jawaharlal Nehru University/IN
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