Recombinant antigen-based immuno-slot blot method for serodiagnosis of syphilis
Braz. j. med. biol. res
;
37(7): 949-955, July 2004. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-360935
RESUMO
Three recombinant antigens of Treponema pallidum Nichols strain were fused with GST, cloned and expressed in Escherichia coli, resulting in high levels of GST-rTp47 and GST-rTp17 expression, and supplementation with arginine tRNA for the AGR codon was needed to obtain GST-rTp15 overexpression. Purified fusion protein yields were 1.9, 1.7 and 5.3 mg/l of cell culture for GST-rTp47, GST-rTp17 and GST-rTp15, respectively. The identities of the antigens obtained were confirmed by automated DNA sequencing using ABI Prism 310 and peptide mapping by Finningan LC/MS. These recombinant antigens were evaluated by immuno-slot blot techniques applied to 137 serum samples from patients with a clinical and laboratory diagnosis of syphilis (61 samples), from healthy blood donors (50 samples), individuals with sexually transmitted disease other than syphilis (3 samples), and from individuals with other spirochetal diseases such as Lyme disease (20 samples) and leptospirosis (3 samples). The assay had sensitivity of 95.1 percent (95 percent CI, 86.1 to 98.7 percent) and a specificity of 94.7 percent (95 percent CI, 87.0 to 98.7 percent); a stronger reactivity was observed with fraction rTp17. The immunoreactivity results showed that fusion recombinant antigens based-immuno-slot blot techniques are suitable for use in diagnostic assays for syphilis.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Treponema pallidum
/
Protéines de fusion recombinantes
/
Sérodiagnostic de la syphilis
/
Syphilis
/
Antigènes bactériens
Type d'étude:
Etude diagnostique
/
Étude observationnelle
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
2004
Type:
Article
/
Congrès et conférence
Pays d'affiliation:
Brésil
/
Japon
Institution/Pays d'affiliation:
Instituto Adolfo Lutz/BR
/
Universidade de São Paulo/BR
/
University of Tokyo/JP
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS