Stage-specific activity of potential antimalarial compounds measured in vitro by flow cytometry in comparison to optical microscopy and hypoxanthine uptake
Mem. Inst. Oswaldo Cruz
;
99(2): 179-184, Mar. 2004. ilus, tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-360972
ABSTRACT
The evaluation of new antimalarial agents using older methods of monitoring sensitivity to antimalarial drugs are laborious and poorly suited to discriminate stage-specific activity. We used flow cytometry to study the effect of established antimalarial compounds, cysteine protease inhibitors, and a quinolone against asexual stages of Plasmodium falciparum. Cultured P. falciparum parasites were treated for 48 h with different drug concentrations and the parasitemia was determined by flow cytometry methods after DNA staining with propidium iodide. P. falciparum erythrocytic life cycle stages were readily distinguished by flow cytometry. Activities of established and new antimalarial compounds measured by flow cytometry were equivalent to results obtained with microscopy and metabolite uptake assays. The antimalarial activity of all compounds was higher against P. falciparum trophozoite stages. Advantages of flow cytometry analysis over traditional assays included higher throughput for data collection, insight into the stage-specificity of antimalarial activity avoiding use of radioactive isotopes.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Plasmodium falciparum
/
Inhibiteurs de la cystéine protéinase
/
Quinolinone
/
Hypoxanthine
/
Antipaludiques
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2004
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Colombie
/
Costa Rica
Institution/Pays d'affiliation:
Universidad Central/VE
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS