Virulence markers and antimicrobial susceptibility of bacteria of the Bacteroides fragilis group isolated from stool of children with diarrhea in São Paulo, Brazil
Mem. Inst. Oswaldo Cruz
;
99(3): 307-312, May 2004. tab
Article
Dans Anglais
| LILACS
| ID: lil-362000
RESUMO
Bacteroides fragilis has been isolated from several human and non-human monomicrobial and mixed infections. In this study, some virulence markers and the antimicrobial susceptibility of bacteria of the B. fragilis group isolated from children's stools were evaluated. All the 64 isolates showed the following characteristics capsulated, beta-hemolytic, hydrophilic, and serum-resistant. Only, 24 (37.5 percent) strains were resistant at 60ºC, for 30 min, and among them, 12 (18.75 percent) were resistant at 60ºC, for 60 min. Also, none strain was resistant at 100ºC. Four strains were able to hemagglutinate erythrocytes and D-mannose, D-galactose, D-arabinose, and D-xylose inhibited hemagglutination in 2 B. fragilis strains (p76a, p76b). The hemagglutination in the strain B. uniformis p3-2 was inhibited by D-xylose and D-galactose. The bft gene detection and the enterotoxin production were observed only in 13 EF-enterotoxigenic species. Fragilysin activity was confirmed on HT-29 cells. The antimicrobial determination confirmed that both imipenem and metronidazole were efficient against B. fragilis species; all the strains were resistant to lead and nickel. Plasmids of 2.9, 4.4, 4.8, and 8.9 kb were observed in 6 tested strains. These results show the values of the species identification from clinical infections, as well as of the periodic evaluation of the resistance patterns of the B. fragilis group at Brazilian medical institutions.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Bacteroides fragilis
/
Infections à Bacteroides
/
Virulence
/
Diarrhée
/
Antibactériens
Type d'étude:
Étude pronostique
Limites du sujet:
Enfant
/
Enfant d'âge préscolaire
/
Humains
/
Bébé
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2004
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade de São Paulo/BR
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