Density and morphometry of myenteric neurons of the ileum of rats subjected to chronic alcoholism

RESUMO

This work verified the changes caused by ethanol ingestion on the myenteric neurons of the ileum of rats subjected to chronic alcoholism. Twenty rats (Rattus norvegicus) were divided in control (GC) and alcoholic (GA) groups during 210 days. The animals from group GA received sugar-cane brandy diluted to 30 GL and those from group GC received water. Both groups were fed with rodent chow ad libitum. The ileum was prepared as whole-mounts for neuronal staining according to Barbosa (1978) and Gabella (1979). The neurons were counted and the cell bodies were measured. The statistical analysis revealed a significant decrease in the number of large neurons in GA compared with GC, while there was a significant increase in the number of small neurons in GA. There was a smaller body growth in GA, thus causing a smaller spread of the nerve cells and a larger number of neurons evidenced by the techniques of Giemsa and NADH-diaphorase in the alcoholic animals than in the controls, and a decrease in the size of the cell bodies, evidenced by the increase in the proportion of small neurons and decrease in the proportion of large nerve cells.