Molecular characterization of Mycobacterium kansasii isolates in the State of São Paulo between 1995-1998
Mem. Inst. Oswaldo Cruz
;
99(7): 739-743, Nov. 2004. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-391604
RESUMO
Mycobacterium kansasii is the most common cause of pulmonary nontuberculous mycobacteria infection and classical identification of this pathogen needs a time consuming phenotypic tests. Polymerase chain reaction-restriction fragment lenght polymorphism analysis (PRA) of the gene enconding for the 65kDa heat shock (hsp65) protein offers an easy, rapid, and inexpensive procedure to identify and subtype M. kansasii isolates. In the present study, we performed a retrospective analysis of patients who had mycobacteria identified on the basis of phenotypic tests by means of a review of database at Mycobacteria Laboratory of the Instituto Adolfo Lutz in the period 1995-1998. A total of 9381 clinical isolates were analyzed of which 7777 (82.9 percent) were identified as M. tuberculosis complex and 1604 (17.1 percent) as nontuberculous mycobacteria. Of the 296 M. kansasii isolates, 189 (63.8 percent) isolates obtained from 119 patients were viable and were analyzed by PRA-hsp65. Hundred eight two (98.9 percent) were classified as M. kansasii type I. Two isolates were classified as type II and III and five isolates were characterized as other Mycobacterium species. Clinical isolates of M. kansasii in the state of São Paulo was almost exclusively subtype I regardless of HIV status.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Mycobacterium kansasii
/
Infections à Mycobacterium
Type d'étude:
Étude observationnelle
Limites du sujet:
Humains
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2004
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Instituto Adolfo Lutz/BR
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