Perfil de sensibilidade a drogas antifúngicas e tipagem molecular de isolados de Pichia anomala provenientes de pacientes com fungemia nosocomial / Susceptibility profile to antifungal drugs and molecular typing of Pichia anomala isolated from patients presenting nosocomial fungemia

RESUMO

Nos últimos anos, as infecções fúngicas têm se caracterizado pelo seu aumento em freqüência e gravidade das infecções, bem como pela diversidade dos fungos isolados. Candida spp representa o quarto grupo de patógenos nosocomiais mais comumente isolados no sangue, porém fungemias causadas por Pichia anomala têm sido descritas com relativa freqüência. No presente estudo, 42 isolados de P. anomala de hemocultura foram analisados. /In the last years, fungal infections have been characterized by increased frequency and gravity of the infections, as well as for the diversity of isolated fungi. Candida spp represents the fourth group of nosocomial pathogens more frequently isolated in peripheral blood. However, fungemias caused by Pichia anomala have been described with relative frequency. In the present study, 42 blood isolates of P. anomala were analyzed. Approximately 100 per cent of them showed susceptibility to fluconazole, voriconazole and amphotericin B, In respect to itraconazole, 32 per cent of the isolates were susceptible, 66 per cent, susceptible-dose dependent and 2 per cent, resistant to this antifungal drug. Electrophoretic karyotyping evidenced different types and subtypes among non- related isolates. Identical karyotype patterns were determined among sequential samples from the same patient and samples from an outbreak. This work presents the greatest number of P. anomala isolates already analyzed in respect to the yeast susceptibility to antifungal agents as well as it demonstrates that electrophoretic karyotyping ...