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A proposal of an alternative in vitro method for the evaluatiopn of the safety of shampoos
Azevedo, Janice C. de; Cruz, Áurea S; Pinto, Terezinha de J. A.
  • Azevedo, Janice C. de; Universidade de São Paulo. São Paulo. BR
  • Cruz, Áurea S; Instituto Adolfo Lutz. BR
  • Pinto, Terezinha de J. A; Universidade de São Paulo. São Paulo. BR
Rev. Inst. Adolfo Lutz ; 62(3): 189-193, 2003. tab
Article Dans Anglais | LILACS, SES-SP | ID: lil-401279
RESUMO
Although tests using animals are still demanded in the evaluation fo shampoos, there is a strong evidence of a tendency for replacing them by in vitro systems. This study aims at defining conditions for the application of a method using NCTC clone 929 cells, as an alternative. Procedures employed to test non diluted samples of shampoos for adults resulted in irritation (in the in vivo method); the same result was not observed when samplesdiluted to a 25 per cent concentration were tested. In the in vitro tests, the same shampoos presented a perfect correlation of results when not diluted (cytotoxic) and when diluted to a 0.1 per cent concentration (non cytotoxic). When the shampoos for children use were tested, the only case of irritation happened with one of the 4 non-diluted shampoos. The absence of cytotoxicity was observed in the in vitro test, after diluting all the samples to 1 per cent and 0.1 per cent. It is possible thus to use the in vitro test instead of the vivo one, if preceded by a careful validation with emphasis on the concentration of the product
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Indice: LILAS (Amériques) Sujet Principal: Techniques in vitro / Santé des Yeux / Tests de toxicité / Produits capillaires langue: Anglais Texte intégral: Rev. Inst. Adolfo Lutz Année: 2003 Type: Article Institution/Pays d'affiliation: Instituto Adolfo Lutz/BR / Universidade de São Paulo/BR

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Indice: LILAS (Amériques) Sujet Principal: Techniques in vitro / Santé des Yeux / Tests de toxicité / Produits capillaires langue: Anglais Texte intégral: Rev. Inst. Adolfo Lutz Année: 2003 Type: Article Institution/Pays d'affiliation: Instituto Adolfo Lutz/BR / Universidade de São Paulo/BR