Typing class I HLA-A gene using a nested PCR-RFLP procedure
Braz. j. med. biol. res
;
38(6): 837-842, June 2005. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-402664
ABSTRACT
In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95 percent. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Test d'histocompatibilité
/
Gènes MHC de classe I
/
Antigènes HLA-A
/
RT-PCR
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
2005
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Estadual Paulista/BR
/
Universidade de São Paulo/BR
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