Combined use of Western blot/ELISA to improve the serological diagnosis of human tuberculosis
Braz. j. infect. dis
;
9(1)Feb. 2005. graf
Article
Dans Anglais
| LILACS
| ID: lil-404306
RESUMO
Two recombinant antigens and a crude bacterial antigen of a wild M. tuberculosis strain were used to detect specific IgG antibodies in sera from 52 patients with pulmonary tuberculosis, confirmed by an acid-fast smear and serum culture of these patients and that of 25 contacts. The patients were not infected with HIV. We evaluated the sensitivity and specificity of ELISA, based on the recombinant TbF6® and TbF6/DPEP antigen and a search for reactivity patterns in the Western blot technique, using whole mycobacterium antigen. Serum samples from 22 healthy individuals and from 30 patients with lung diseases other than tuberculosis were used as controls. The best ELISA results were obtained with the TbF6/DPEP antigen combination, which gave 85 percent sensitivity and 91 percent specificity. ELISA sensitivity improved from 85 percent to 92 percent when the Western blot results were used. Western blot specificity was 100 percent when antibody reactivity with different antigenic bands was analyzed and associated. The association of TbF6/DPEP antigens used in ELISA with specific patterns of reactivity determined by Western blot can help make an identification when classic methods for the diagnosis of pulmonary tuberculosis are not sufficient.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Tuberculose pulmonaire
/
Anticorps antibactériens
/
Mycobacterium tuberculosis
/
Antigènes bactériens
Type d'étude:
Etude diagnostique
/
Étude observationnelle
/
Étude pronostique
/
Facteurs de risque
Limites du sujet:
Adolescent
/
Adulte
/
Adulte très âgé
/
Humains
langue:
Anglais
Texte intégral:
Braz. j. infect. dis
Thème du journal:
Maladies transmissibles
Année:
2005
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
A+BR
/
Clinical Hospital/BR
/
Federal University of Santa Maria/BR
/
São Paulo University/BR
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