Preliminary study of the presence of antibodies against excretory-secretory antigens from protoscoleces of Echinococcus granulosus in dogs with intestinal echinococcosis
Mem. Inst. Oswaldo Cruz
;
100(3): 311-317, May 2005. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-411031
ABSTRACT
The aim of the present study was to analyze the antibody response against excretory-secretory antigens (ES-Ag) from Echinococcus granulosus protoscoleces, using sera from dogs infected with E. granulosus and other helminths. ES-Ag were obtained from the first 50 h maintenance of protoscoleces in vitro. Immunochemical characterization was performed by immunoblotting with sera from dogs naturally infected with E. granulosus (n = 12), sera from dogs infected with helminths other than E. granulosus (n = 30), and helminth-free dog sera (n = 20). These findings were compared to those obtained from a somatic extract of protoscoleces (S-Ag). ES-Ag only showed four cross-reacting proteins of 65, 61, 54, and 45-46 kDa. Antigens with apparent masses of 89 and 50 kDa in ES-Ag and of 130 and 67 kDa in S-Ag were identified by sera of dogs infected with E. granulosus only, whereas a protein of 41-43 kDa was recognised by the majority of the sera from dogs with non-echinococcal infection. Employing ELISA to study the same sera, S-Ag revealed higher immunoreactivity than ES-Ag, but also showed higher cross-reactivity levels when sera from dogs with non-echinococcal infection were assayed in immunoblotting.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Echinococcus granulosus
/
Maladies des chiens
/
Échinococcose
/
Parasitoses intestinales
/
Antigènes d'helminthe
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2005
Type:
Article
Pays d'affiliation:
Espagne
Institution/Pays d'affiliation:
Universidad del País Vasco/ES
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