Birth of normal kids after microinjection of pronuclear embryos in a transgenic goat (Capra hircus) production program in Brazil
Genet. mol. res. (Online)
;
2(2): 200-205, Jun. 2003.
Article
Dans Anglais
| LILACS
| ID: lil-417609
ABSTRACT
This pilot project was designed to determine if normal kids could be produced after microinjection in pronuclear embryos and subsequent transfer to recipients in a transgenic goat program in Brazil. Twelve donors of the Saanen breed and 17 recipients of an undefined breed were used. The estrus of both donors and recipients was synchronized by a standard progestagen treatment and superovulation obtained by six pFSH injections. Donors in estrus were mated with fertile Saanen bucks. Zygotes were recovered surgically by flushing oviducts. The recovered zygotes with visible pronuclei were microinjected with 500 to 1000 copies of the human G-CSF gene. Two or four embryos were surgically transferred into the oviducts of recipients. One recipient became pregnant and two kids were born. No transgenic goat was identified after PCR analysis. Even though transgenic goats were not obtained, this experiment establishes the basis of a synchronization and superovulation regimen for use in goats raised in Brazil, for the purpose of collecting and manipulating the pronuclear embryos. This project also showed that microinjected one-cell goat embryos can survive to produce live young following surgical transfer
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Zygote
/
Capra
/
Animal génétiquement modifié
/
Facteur de stimulation des colonies de granulocytes
/
Transfert d'embryon
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
/
Grossesse
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Genet. mol. res. (Online)
Thème du journal:
Biologie moléculaire
/
Génétique
Année:
2003
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
State University of Ceará/BR
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