Development of enzyme-linked immunosorbent assays based on recombinant MSP1a and MSP2 of Anaplasma marginale
Mem. Inst. Oswaldo Cruz
;
100(7): 765-769, Nov. 2005. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-419704
ABSTRACT
Indirect enzyme-linked immunosorbent assays (ELISAs) based on recombinant MSP1a and MSP2 from a Brazilian isolate of Anaplasma marginale were developed to detect antibodies against this rickettsia in cattle. The high sensitivities (99 percent for both tests) and specificities (100 percent for both tests) were confirmed with sera from cattle positive or negative for A. marginale antibodies, respectively, by immunofluorescent antibody test. By the analysis of 583 sera from cattle of three regions of the state of Pernambuco, Brazil, the agreement between both tests was high, with a kappa index of 0.89. The similar performances of the ELISAs suggest that both tests can be used in epidemiological surveys for detection of antibodies to A. marginale in cattle.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Protéines de la membrane externe bactérienne
/
Test ELISA
/
Maladies des bovins
/
Anaplasma marginale
/
Anaplasmose
/
Anticorps antibactériens
/
Antigènes bactériens
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2005
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Embrapa Gado de Corte/BR
/
Instituto de Biologia Molecular do Paraná/BR
/
Universidade Federal Rurual de Pernambuco/BR
/
Universidade Federal da Bahia/BR
/
Universidade Federal do Mato Grosso do Sul/BR
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