Intracellular polyamine pools, oligopeptide-binding protein A expression, and resistance to aminoglycosides in Escherichia coli
Mem. Inst. Oswaldo Cruz
;
100(7): 789-793, Nov. 2005. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-419708
RESUMO
The role of intracellular free polyamine (putrescine and spermidine) pools in multiple resistance to aminoglycoside antibiotics was investigated among in vitro selected kanamycin-resistant Escherichia coli J53 mutants expressing diminished oligopeptide-binding protein (OppA) levels and/or defective ornithine decarboxylase (ODC) activity. The results suggest that diminished OppA content, but not defective ODC activity expression, increased the relative concentration of free spermidine as compared to the wild type strain. Moreover, by adding exogenous polyamines or polyamine synthesis inhibitors to cultures with different mutant strains, a direct relationship between the intracellular OppA levels and resistance to kanamycin was revealed. Collectively these results further suggest a complex relation among OppA expression, aminoglycoside resistance and polyamine metabolism.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Polyamines
/
Protéines bactériennes
/
Multirésistance bactérienne aux médicaments
/
Escherichia coli
/
Aminosides
/
Antibactériens
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2005
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Católica de Santos/BR
/
Universidade de São Paulo/BR
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