Gene expression profiling of clinical stages II and III breast cancer
Braz. j. med. biol. res
;
39(8): 1101-1113, Aug. 2006. tab
Article
Dans Anglais
| LILACS
| ID: lil-433168
RESUMO
Clinical stage (CS) is an established indicator of breast cancer outcome. In the present study, a cDNA microarray platform containing 692 genes was used to identify molecular differences between CSII and CSIII disease. Tumor samples were collected from patients with CSII or CSIII breast cancer, and normal breast tissue was collected from women without invasive cancer. Seventy-eight genes were deregulated in CSIII tumors and 22 in CSII tumors when compared to normal tissue, and 20 of them were differentially expressed in both CSII and CSIII tumors. In addition, 58 genes were specifically altered in CSIII and expression of 6 of them was tested by real time RT-PCR in another cohort of patients with CSII or CSIII breast cancer and in women without cancer. Among these genes, MAX, KRT15 and S100A14, but not APOBEC3G or KRT19, were differentially expressed on both CSIII and CSII tumors as compared to normal tissue. Increased HMOX1 levels were detected only in CSIII tumors and may represent a molecular marker of this stage. A clear difference in gene expression pattern occurs at the normal-to-cancer transition; however, most of the differentially expressed genes are deregulated in tumors of both CS (II and III) compared to normal breast tissue.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Tumeurs du sein
/
Régulation de l'expression des gènes tumoraux
/
Analyse de profil d'expression de gènes
Type d'étude:
Étude observationnelle
Limites du sujet:
Adulte
/
Adulte très âgé
/
Femelle
/
Humains
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
2006
Type:
Article
/
Congrès et conférence
/
descriptif de projet
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Hospital Amaral Carvalho/BR
/
Hospital do Câncer A. C. Camargo/BR
/
Hospital do Câncer A. C.Camargo/BR
/
Hospital do Câncer A.C/BR
/
Instituto Brasileiro de Controle do Câncer/BR
/
Instituto Ludwig de Pesquisa sobre o Câncer/BR
/
Universidade de São Paulo/BR
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