Diagnosis of human papillomatosis by polymerase chain reaction in cases of divergence between results of hybrid capture and papanicolaou cytology
Braz. j. infect. dis
;
10(3): 169-172, June 2006. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-435281
ABSTRACT
As various types of human papillomavirus (HPV) are involved in the pathogenesis of cervical cancer, correct diagnosis is of fundamental importance for screening programs. We evaluated the divergence of results between Papanicolaou cytology and hybrid capture by PCR detection of HPV DNA . A transversal study was conducted on 70 women attending private gynecological clinics in Brasilia, Brazil. PCRs were conducted with specific primers for general and high-risk HPV DNA. Based on the PCR results, hybrid capture was a superior diagnostic technique. When Papanicolaou was compared with the molecular biology methods, it was found that a positive Papanicolaou result does not necessarily indicate the presence of HPV. The agreement between PCR and hybrid capture results can be attributed to the fact that both methods detect latent infection, while Papanicolaou detects only microscopic cellular alterations.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Papillomaviridae
/
Frottis vaginaux
/
ADN viral
/
Réaction de polymérisation en chaîne
/
Infections à papillomavirus
Type d'étude:
Etude diagnostique
/
Étude observationnelle
/
Étude de prévalence
/
Facteurs de risque
Limites du sujet:
Adulte
/
Adulte très âgé
/
Femelle
/
Humains
langue:
Anglais
Texte intégral:
Braz. j. infect. dis
Thème du journal:
Maladies transmissibles
Année:
2006
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Catholic University of Brasília/BR
/
School of Meical Health/BR
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