Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
Mem. Inst. Oswaldo Cruz
;
101(5): 499-501, Aug. 2006. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-437033
ABSTRACT
A multiplex polymerase chain reaction (PCR) assay was performed on 167 thermophilic campylobacters isolated from non-human primates. Samples were first identified by phenotypic methods resulting in 64 Campylobacter jejuni and 103 C. coli strains. Four strains identified biochemically as C. coli, were then determined to be C. jejuni by PCR. Comparison of methodologies showed that the main discrepancies were attributed to the hippurate hydrolysis test and sensitivity to cephalothin and nalidixic acid. Analysis of data showed that the application of phenotypic methods should be supplemented by a molecular method to offer a more reliable Campylobacter identification.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Campylobacter
/
ADN bactérien
/
Réaction de polymérisation en chaîne
/
Techniques de typage bactérien
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2006
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Fundação Oswaldo Cruz/BR
/
Universidade Federal do Rio de Janeiro/BR
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