Protein kinase C isoform specificity of cholinergic potentiation of glucose-induced pulsatile 5-HT/ insulin release from mouse pancreatic islets
Biol. Res
;
39(3): 531-539, 2006. ilus
Article
Dans Anglais
| LILACS
| ID: lil-437385
ABSTRACT
Thymeleatoxin (TMX), an activator of Ca2+-sensitive protein kinase C (cPKC) isoforms, was used to assess the PKC isoform specificity of cholinergic potentiation of glucose (11 mM)-induced pulsatile 5-HT/insulin release (PIR) from single mouse pancreatic islets. TMX (100 nM) and carbachol (Cch, 50 mM) enhanced PIR ~ 3-fold while reducing the underlying [Ca2+]i oscillations (duration and amplitude) by ~ 40-50 percent. Both effects were ablated by the specific PKC inhibitor bisindolylmaleimide and chronic TMX pretreatment. Cch also evoked an initial transient [Ca2+]i rise and surge of 5-HT release, which remained unaffected by chronic TMX pretreatment. It is concluded that the immediate cholinergic responses are insensitive to cPKC. In contrast, specific activation of a cPKC isoform mediates sustained cholinergic potentiation of glucose-induced insulin secretion.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Protéine kinase C
/
Esters de phorbol
/
Sérotonine
/
Ilots pancréatiques
/
Glucose
/
Insuline
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Biol. Res
Thème du journal:
Biologie
Année:
2006
Type:
Article
Pays d'affiliation:
Portugal
Institution/Pays d'affiliation:
University of Coimbra/PT
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS