Imaging single-channel calcium microdomains by total internal reflection microscopy
Biol. Res
;
37(4): 675-679, 2004. ilus, graf
Article
Dans Anglais
| LILACS
| ID: lil-437525
ABSTRACT
The microdomains of Ca2+ in the cytosol around the mouth of open Ca2+ channels are the basic `building blocks' from which cellular Ca2+ signals are constructed. Moreover, the kinetics of local [Ca2+] closely reflect channel gating, so their measurement holds promise as an alternative to electrophysiological patch-clamp recording as a means to study single channel behavior. We have thus explored the development of optical techniques capable of imaging single-channel Ca2+ signals with good spatial and temporal resolution, and describe results obtained using total internal reflection fluorescence microscopy to monitor Ca2+ influx through single N-type channels expressed in Xenopus oocytes.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Canaux calciques
/
Échangeur sodium-calcium
/
Canaux ioniques
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Biol. Res
Thème du journal:
Biologie
Année:
2004
Type:
Article
Pays d'affiliation:
États-Unis d'Amérique
Institution/Pays d'affiliation:
University of California/US
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