The post-transcriptional gene silencing pathway in Eucalyptus
Genet. mol. biol
;
28(3,suppl): 496-500, Nov. 2005. ilus, tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-440461
ABSTRACT
Post-transcriptional gene silencing (PTGS) is a conserved surveillance mechanism that identifies and cleaves double-stranded RNA molecules and their cellular cognate transcripts. The RNA silencing response is actually used as a powerful technique (named RNA interference) for potent and specific inhibition of gene expression in several organisms. To identify gene products in Eucalyptus sharing similarities with enzymes involved in the PTGS pathway, we queried the expressed sequence tag database of the Brazilian Eucalyptus Genome Sequence Project Consortium (FORESTs) with the amino acid sequences of known PTGS-related proteins. Among twenty-six prospected genes, our search detected fifteen assembled sequences encoding products presenting high level of similarity (E value < 10-40) to proteins involved in PTGS in plants and other organisms. We conclude that most of the genes known to be involved in the PTGS pathway are represented in the FORESTs database
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Plantes
/
Extinction de l'expression des gènes
/
Eucalyptus
langue:
Anglais
Texte intégral:
Genet. mol. biol
Thème du journal:
Génétique
Année:
2005
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Estadual Paulista "Júlio de Mesquita Filho/BR
/
Universidade Estadual de Campinas/BR
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