Identificación de asociaciones clínico-patológicas e hipermetilación de genes supresores de tumores en cáncer gástrico difuso a través de análisis de Hierarchical clustering / Hierarchical clustering analysis to detect associations between clinical and pathological features of gastric tumors and hypermethylation of suppressor genes
Rev. méd. Chile
;
135(1): 17-25, ene. 2007. ilus, tab, graf
Article
Dans Espagnol
| LILACS
| ID: lil-442997
ABSTRACT
Background:
Methylation is an inactivation mechanism for tumor suppressor genes, that can have important clinical implications.Aim:
To analyze the methylation status of 11 tumor suppressor genes in pathological samples of diffuse gastric cancer. Material andmethods:
Eighty three patients with diffuse gastric cancer with information about survival and infection with Epstein Barr virus, were studied. DNA was extracted from pathological slides and the methylation status of genes p14, p15, p16, APC, p73, FHIT, E-caderin, SEMA3B, BRCA-1, MINT-2 y MGMT, was studied using sodium bisulphite modification and polymerase chain reaction. Results were grouped according to the methylation index or Hierarchical clustering (TIGR MultiExperiment Viewer).Results:
Three genes had a high frequency of methylation (FHIT, BRCA1, APC), four had an intermediate frequency (p15, MGMT, p14, MINT2) and four had a low frequency (p16, p73, E-cadherin, SEMA3B). The methylation index had no association with clinical or pathological features of tumors or patients survival. Hierarchical clustering generated two clusters. One grouped clinical and pathological features with FHIT, BRCA1, and APC and the other grouped the other eight genes and Epstein Barr virus infection. Two significant associations were found, between APC and survival and p16/p14 and Epstein Barr virus infection.Conclusions:
Hierarchical clustering is a tool that identifies associations between clinical and pathological features of tumors and methylation of tumor suppressor genes.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Tumeurs de l'estomac
/
Carcinomes
/
Gènes suppresseurs de tumeur
/
Régions promotrices (génétique)
/
Méthylation de l'ADN
Type d'étude:
Etude diagnostique
/
Facteurs de risque
Limites du sujet:
Adulte
/
Adulte très âgé
/
Aged80
/
Femelle
/
Humains
/
Mâle
langue:
Espagnol
Texte intégral:
Rev. méd. Chile
Thème du journal:
Médicament
Année:
2007
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Chili
Institution/Pays d'affiliation:
Hospital Clínico-San Borja Arriarán/CL
/
Pontificia Universidad Católica de Chile/CL
/
Universidad de Chile/CL
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