Isolation and molecular identification of Leishmania ( Viannia ) peruviana from naturally infected Lutzomyia peruensis (Diptera: Psychodidae) in the Peruvian Andes
Mem. Inst. Oswaldo Cruz
;
102(5): 655-658, Aug. 2007. ilus
Article
Dans Anglais
| LILACS
| ID: lil-458635
ABSTRACT
Leishmania (Viannia) peruviana was isolated from 1/75 Lutzomyia peruensis captured during May 2006 in an endemic cutaneous leishmaniasis region of the Peruvian Andes (Chaute, Huarochiri, Lima, Peru). Sand fly gut with promastigotes was inoculated into a hamster and the remaining body was fixed in ethanol. L. (Viannia) sp. was determined by polymerase chain reaction (PCR), and Leishmania species through molecular genotyping by PCR-restriction fragment length polymorphism analyses targeting the genes cpb and hsp70, resulting L. (V.) peruviana. The infected sand fly appeared 15 days after the rains finished, time expected and useful real time data for interventions when transmission is occurring.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Psychodidae
/
Leishmania brasiliensis
/
ADN des protozoaires
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
Pays comme sujet:
Amérique du Sud
/
Pérou
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2007
Type:
Article
Pays d'affiliation:
Pérou
Institution/Pays d'affiliation:
Universidad Peruana Cayetano Heredia/PE
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