Mapping the molecular characteristics of Brazilian human T-cell lymphotropic virus type 1 Env (gp46) and Pol amino acid sequences for vaccine design
Mem. Inst. Oswaldo Cruz
;
102(6): 741-749, Sept. 2007. tab
Article
Dans Anglais
| LILACS
| ID: lil-463482
ABSTRACT
This study was carried out to evaluate the molecular pattern of all available Brazilian human T-cell lymphotropic virus type 1 Env (n = 15) and Pol (n = 43) nucleotide sequences via epitope prediction, physico-chemical analysis, and protein potential sites identification, giving support to the Brazilian AIDS vaccine program. In 12 previously described peptides of the Env sequences we found 12 epitopes, while in 4 peptides of the Pol sequences we found 4 epitopes. The total variation on the amino acid composition was 9 and 17 percent for human leukocyte antigen (HLA) class I and class II Env epitopes, respectively. After analyzing the Pol sequences, results revealed a total amino acid variation of 0.75 percent for HLA-I and HLA-II epitopes. In 5 of the 12 Env epitopes the physico-chemical analysis demonstrated that the mutations magnified the antigenicity profile. The potential protein domain analysis of Env sequences showed the loss of a CK-2 phosphorylation site caused by D197N mutation in one epitope, and a N-glycosylation site caused by S246Y and V247I mutations in another epitope. Besides, the analysis of selection pressure have found 8 positive selected sites (w = 9.59) using the codon-based substitution models and maximum-likelihood methods. These studies underscore the importance of this Env region for the virus fitness, for the host immune response and, therefore, for the development of vaccine candidates.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Vaccins antiviraux
/
Conception de médicament
/
Virus T-lymphotrope humain de type 1
/
Produits du gène env
/
Protéines oncogènes des retroviridae
/
Cartographie épitopique
Type d'étude:
Étude pronostique
Limites du sujet:
Humains
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2007
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Brésil
/
Royaume-Uni
Institution/Pays d'affiliation:
Faculdade de Tecnologia e Ciência/BR
/
Fiocruz/BR
/
South African National Bioinformatics Institute/GB
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