Immunoelectronic localization of myosin-Va in rat cerebellum
Braz. j. morphol. sci
;
22(4): 211--214, Oct.-Dec. 2005. ilus
Article
Dans Anglais
| LILACS
| ID: lil-468028
ABSTRACT
Myosin V is an unconventional type of actin-binding myosin that participates in cytoplasmic organelle transport. Although this unconventional myosin has been extensively studied, its subcellular localization in the mammalian cerebellum has not been determined. In this work, we used an antibody against the tail domain of the myosin-Va heavy chain and a secondary antibody labeled with protein A-gold (15 nm) to study the subcellular distribution of this protein. Myosin-Va was found in the cytoplasm, where it was associated with a filament (probably actin). This protein was also detected in the plasma membrane of axons and dendrites in the molecular layer in rat cerebellum.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Cellules de Purkinje
/
Protéine A staphylococcique
/
Télencéphale
/
Myosine de type V
/
Phénomènes physiologiques du système nerveux
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Braz. j. morphol. sci
Thème du journal:
Anatomie
Année:
2005
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Federal University of Goiás/BR
/
Federal University of Uberlândia/BR
/
University of Patos de Minas/BR
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