The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle
Biol. Res
;
40(2): 185-192, 2007. ilus
Article
Dans Anglais
| LILACS
| ID: lil-468189
ABSTRACT
We used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the region surrounding two overlapping elements, an origin of bidirectional DNA replication and the GAS41 promoter, in chicken myelomonocytic HD11 cells arrested in G0, G0 and S phases as well as at the G0/S border. The results show that the chromatin of this region became more nuclease sensitive when cells were arrested in G0 phase and that this change in chromatin structure was reversible after the cells began to enter S phase.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Facteurs de transcription
/
Chromatine
/
Cycle cellulaire
/
Réplication de l'ADN
Type d'étude:
Guide de pratique
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Biol. Res
Thème du journal:
Biologie
Année:
2007
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Allemagne
Institution/Pays d'affiliation:
Federal Agricultural Research Centre/DE
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