Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
J. venom. anim. toxins incl. trop. dis
;
13(4): 874-880, 2007. ilus
Article
Dans Anglais
| LILACS
| ID: lil-471147
ABSTRACT
A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Plasmides
/
Virus de la rage
/
Protéines de fusion recombinantes
/
Glycoprotéines
/
Transfection
langue:
Anglais
Texte intégral:
J. venom. anim. toxins incl. trop. dis
Thème du journal:
Toxicologie
Année:
2007
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Pasteur Institute/BR
/
University of São Paulo/BR
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