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Experimental rat lung tumor model with intrabronchial tumor cell implantation
Gomes Neto, Antero; Simão, Antônio Felipe Leite; Miranda, Samuel de Paula; Mourão, Lívia Talita Cajaseiras; Bezerra, Nilfácio Prado; Almeida, Paulo Roberto Carvalho de; Ribeiro, Ronaldo de Albuquerque.
  • Gomes Neto, Antero; Federal University of Ceará. Fortaleza. BR
  • Simão, Antônio Felipe Leite; Federal University of Ceará. Fortaleza. BR
  • Miranda, Samuel de Paula; Federal University of Ceará. Fortaleza. BR
  • Mourão, Lívia Talita Cajaseiras; Federal University of Ceará. Fortaleza. BR
  • Bezerra, Nilfácio Prado; Federal University of Ceará. Fortaleza. BR
  • Almeida, Paulo Roberto Carvalho de; Federal University of Ceará. Department of Pathology. Fortaleza. BR
  • Ribeiro, Ronaldo de Albuquerque; Federal University of Ceará. Department of Surgery. Department of Physiology and Pharmacology. Fortaleza. BR
Acta cir. bras ; 23(1): 84-92, Jan.-Feb. 2008. ilus, graf
Article Dans Anglais | LILACS | ID: lil-474145
ABSTRACT

PURPOSE:

The objective of this study was to develop a rat lung tumor model for anticancer drug testing.

METHODS:

Sixty-two female Wistar rats weighing 208 ± 20 g were anesthetized intraperitoneally with 2.5 percent tribromoethanol (1 ml/100 g live weight), tracheotomized and intubated with an ultrafine catheter for inoculation with Walker's tumor cells. In the first step of the experiment, a technique was established for intrabronchial implantation of 10(5) to 5×10(5) tumor cells, and the tumor take rate was determined. The second stage consisted of determining tumor volume, correlating findings from high-resolution computed tomography (HRCT) with findings from necropsia and determining time of survival.

RESULTS:

The tumor take rate was 94.7 percent for implants with 4×10(5) tumor cells, HRCT and necropsia findings matched closely (r=0.953; p<0.0001), the median time of survival was 11 days, and surgical mortality was 4.8 percent.

CONCLUSION:

The present rat lung tumor model was shown to be feasible the take rate was high, surgical mortality was negligible and the procedure was simple to perform and easily reproduced. HRCT was found to be a highly accurate tool for tumor diagnosis, localization and measurement and may be recommended for monitoring tumor growth in this model.
RESUMO

OBJETIVO:

O objetivo foi desenvolver um modelo de tumor de pulmão em rato que permita o teste de fármacos no tratamento deste câncer.

MÉTODOS:

Sessenta e dois ratos Wistar fêmeas, peso médio de 208±20 g, foram anestesiados com tribromo-etanol 2,5 por cento IP (1ml/100g de rato), traqueostomizados e intubados com cateter ultrafino para injetar células do tumor de Walker. Na 1ª etapa, estabeleceu-se a técnica do implante de células tumorais por via intrabrônquica e o índice de pega tumoral, usando-se de 10(5) a 5×10(5) células. Na 2ª, avaliou-se o volume tumoral e a correlação dos achados obtidos na tomografia computadorizada de alta resolução (TCAR) de tórax com os da necropsia e verificou-se a sobrevida.

RESULTADOS:

O índice de pega foi de 94,7, com o implante de 4×10(5) células do tumor; as medidas do tumor feitas na TCAR e comparadas com as da necropsia foram semelhantes (r=0, 953, p<0,0001); a sobrevida mediana foi de 11 dias; e a mortalidade cirúrgica de 4,8 por cento.

CONCLUSÃO:

O modelo mostrou-se viável, com alto índice de pega, mortalidade cirúrgica desprezível, de execução simples e fácil reprodutibilidade. A TCAR revelou alta acurácia no diagnóstico, localização e mensuração das lesões tumorais, credenciando-se para a monitorização de crescimento tumoral nesse modelo.
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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Tumeurs du poumon Type d'étude: Étude pronostique Limites du sujet: Animaux langue: Anglais Texte intégral: Acta cir. bras Thème du journal: Chirurgie générale / Procedimentos Cir£rgicos Operat¢rios Année: 2008 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Federal University of Ceará/BR

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Tumeurs du poumon Type d'étude: Étude pronostique Limites du sujet: Animaux langue: Anglais Texte intégral: Acta cir. bras Thème du journal: Chirurgie générale / Procedimentos Cir£rgicos Operat¢rios Année: 2008 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Federal University of Ceará/BR