Your browser doesn't support javascript.
loading
Effects of pentoxifylline on TNF-alpha and lung histopathology in HCL-induced lung injury
Oliveira-Júnior, Itamar Souza de; Maganhin, Carla Cristina; Carbonel, Adriana Aparecida Ferraz; Monteiro, Cristina Maria Rodrigues; Cavassani, Sâmia Santos; Oliveira-Filho, Ricardo Martins.
  • Oliveira-Júnior, Itamar Souza de; Federal University of São Paulo. Department of Surgery. Surgery Center. São Paulo. BR
  • Maganhin, Carla Cristina; Federal University of São Paulo. Department of Surgery. Surgery Center. São Paulo. BR
  • Carbonel, Adriana Aparecida Ferraz; Federal University of São Paulo. Department of Surgery. Surgery Center. São Paulo. BR
  • Monteiro, Cristina Maria Rodrigues; Federal University of São Paulo. Department of Surgery. Surgery Center. São Paulo. BR
  • Cavassani, Sâmia Santos; Federal University of São Paulo. Department of Surgery. Surgery Center. São Paulo. BR
  • Oliveira-Filho, Ricardo Martins; Federal University of São Paulo. Department of Surgery. Surgery Center. São Paulo. BR
Clinics ; 63(1): 77-84, 2008. ilus, graf, tab
Article Dans Anglais | LILACS | ID: lil-474932
ABSTRACT

OBJECTIVE:

To evaluate the effects of pentoxifylline on hydrochloric acid-induced lung lesions in rats subjected to mechanical ventilation.

METHODS:

Twenty male, adult Wistar-EPM-1 rats were anesthetized and randomly grouped (n=5 animals per group) as follows control-MV (mechanical ventilation, MV group); bilateral instillation of HCl (HCl group); bilateral instillation of HCl followed by pentoxifylline (50 mg/kg bw) infusion (HCl+PTX group) and pentoxifylline infusion followed by bilateral instillation of HCl (PTX+HCl group). At 20, 30, 90 and 180 min after treatments, the blood partial pressures of CO2 and O2 were measured. The animals were euthanized, and bronchoalveolar lavages were taken to determine the contents of total proteins, corticosteroid and TNF-alpha. Samples of lung tissue were used for histomorphometric studies and determining the wet-to-dry (W/D) lung weight ratio.

RESULTS:

In the MV group, rats had alveolar septal congestion, and, in the HCl group, a remarkable recruitment of neutrophils and macrophages into the alveoli was noticed; these events were reduced in the animals with PTX+HCl. The partial pressure of oxygen increased in PTX+HCl animals (121±5 mmHg) as compared with the HCl (62±6 mmHg) and HCl+PTX (67±3 mmHg) groups within 30 minutes. TNF-alpha levels in bronchoalveolar lavage were significantly higher in the HCl group (458±50 pg/mL), reduced in the HCl+PTX group (329±45 pg/mL) and lowest in the PTX+HCl group (229±41 pg/mL). The levels of corticosteroid in bronchoalveolar lavage were significantly lower in the HCl (8±1.3 ng/mL) and HCl+PTX group (16±2 ng/mL) and were highest in the PTX+HCl (27±1.9 ng/mL).

CONCLUSION:

Pretreatment with PTX improves oxygenation, reduces TNF-alpha concentration and increases the concentration of corticosteroid in bronchoalveolar lavage upon lung lesion induced by HCl.
Sujets)

Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Pentoxifylline / Liquide de lavage bronchoalvéolaire / Facteur de nécrose tumorale alpha / Lésion pulmonaire / Poumon Limites du sujet: Animaux langue: Anglais Texte intégral: Clinics Thème du journal: Médicament Année: 2008 Type: Article / descriptif de projet Pays d'affiliation: Brésil Institution/Pays d'affiliation: Federal University of São Paulo/BR

Documents relatifs à ce sujet

MEDLINE

...
LILACS

LIS

Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Pentoxifylline / Liquide de lavage bronchoalvéolaire / Facteur de nécrose tumorale alpha / Lésion pulmonaire / Poumon Limites du sujet: Animaux langue: Anglais Texte intégral: Clinics Thème du journal: Médicament Année: 2008 Type: Article / descriptif de projet Pays d'affiliation: Brésil Institution/Pays d'affiliation: Federal University of São Paulo/BR