Analysis of blood processing conditions to obtain high-quality total RNA from human leukocyte concentrate
Genet. mol. res. (Online)
;
6(2): 298-307, 2007. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-482040
ABSTRACT
Blood samples are used as a biological source to discover biomarkers of hematological and non-hematological disorders. The present study shows the impact of different experimental conditions associated with cell lysis buffer, TRI-reagent protocol and blood cell storage buffer and their correlation with the quantity, quality and Adrenomedullin gene expression levels of total RNA when RT-PCR technique is used. A leukocyte cell bank protocol is also proposed for further mRNA expression analysis using RNAlater as storage buffer. There is evidence that total RNA isolated from leukocyte concentrate stored for 1 month at -70 degrees C did not show significant differences concerning quality, purity and Adrenomedullin gene expression compared with the freshly processed leukocyte sample.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Conservation de sang
/
ARN
/
ARN messager
/
Leucocytes
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Genet. mol. res. (Online)
Thème du journal:
Biologie moléculaire
/
Génétique
Année:
2007
Type:
Article
Pays d'affiliation:
Cuba
Institution/Pays d'affiliation:
Center for Genetic Engineering and Biotechnology/CU
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS