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Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification
Marassá, A. M; Rosa, M. D. B; Gomes, A. C; Consales, C. A.
  • Marassá, A. M; Adolfo Lutz Institute. São Paulo. BR
  • Rosa, M. D. B; University of São Paulo. School of Public Health. Department of Epidemiology. São Paulo. BR
  • Gomes, A. C; University of São Paulo. School of Public Health. Department of Epidemiology. São Paulo. BR
  • Consales, C. A; Pasteur Institute. São Paulo. BR
J. venom. anim. toxins incl. trop. dis ; 14(2): 303-312, 2008. graf
Article Dans Anglais | LILACS | ID: lil-484566
ABSTRACT
The knowledge of mosquitoes Culicidae host feeding patterns is basic to understand the roles of different species and to indicate their importance in the epidemiology of arthropod-borne diseases. A laboratory assay was developed aiming at standardizing the biotin-avidin sandwich enzyme-linked immunosorbent assay, which was unprecedented for mosquito blood meal identification. The enzyme-linked immunosorbent assay (ELISA) activity was evaluated by the detection of titers on each sample of the 28 blood-fed Culex quinquefasciatus. In light of the high sensitivity that the technique permits, by means of small quantities of specific antibodies commercially provided and phosphatase substrate which reinforces additional dilutions, human and rat blood meals were readily identified in all laboratory-raised Culex quinquefasciatus tested. The assay was effective to detect human blood meal dilutions up to 14,096, which enables the technique to be applied in field studies. Additionally, the present results indicate a significant difference between the detection patterns recorded from human blood meal which corroborate the results of host feeding patterns.
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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Biotine / Avidine / Culicidae Type d'étude: Etude diagnostique Limites du sujet: Animaux langue: Anglais Texte intégral: J. venom. anim. toxins incl. trop. dis Thème du journal: Toxicologie Année: 2008 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Adolfo Lutz Institute/BR / Pasteur Institute/BR / University of São Paulo/BR

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Biotine / Avidine / Culicidae Type d'étude: Etude diagnostique Limites du sujet: Animaux langue: Anglais Texte intégral: J. venom. anim. toxins incl. trop. dis Thème du journal: Toxicologie Année: 2008 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Adolfo Lutz Institute/BR / Pasteur Institute/BR / University of São Paulo/BR