Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil
Mem. Inst. Oswaldo Cruz
;
103(7): 731-733, Nov. 2008. tab
Article
Dans Anglais
| LILACS
| ID: lil-498384
ABSTRACT
The incidence of Shigella spp. was assessed in 877 infants from the public hospital in Rondônia (Western Amazon region, Brazil) where Shigella represents the fourth cause of diarrhea. Twenty-five isolates were identified 18 were Shigella flexneri, three Shigella sonnei, three Shigella boydii and one Shigella dysenteriae. With the exception of S. dysenteriae, all Shigella spp. isolated from children with diarrhea acquired multiple antibiotic resistances. PCR detection of ipa virulence genes and invasion assays of bloody diarrhea and fever (colitis) were compared among 25 patients testing positive for Shigella. The ipaH and ipaBCD genes were detected in almost all isolates and, unsurprisingly, all Shigella isolates associated with colitis were able to invade HeLa cells. This work alerts for multiple antibiotic resistant Shigella in the region and characterizes presence of ipa virulence genes and invasion phenotypesin dysenteric shigellosis.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Shigella
/
Colite
/
Diarrhée
/
Dysenterie bacillaire
Type d'étude:
Etude diagnostique
/
Etude d'incidence
/
Étude pronostique
Limites du sujet:
Enfant d'âge préscolaire
/
Humains
/
Bébé
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2008
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Centro de Pesquisa em Medicina Tropical/BR
/
Fiocruz/BR
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