Functional complementation in Saccharomyces cerevisiae under control of the natural yeast promoter
Electron. j. biotechnol
;
10(2): 322-327, Apr. 15, 2007. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-499171
ABSTRACT
A novel approach for functional complementation of foreign genes in Saccharomyces cerevisiae is presented. This approach is based on the use of the widely available cognate gene plasmids (e.g. pRS416) of the European Functional Analysis Network (EUROFAN). The functional complementation of the human homolog of YOR159c (SME1 gene) shown here is the first demonstration of complementation using the original yeast promoter, theoretically offering a more natural regulation of protein expression
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Plasmides
/
Saccharomyces cerevisiae
/
Levures
/
Gènes fongiques
/
Test de complémentation
langue:
Anglais
Texte intégral:
Electron. j. biotechnol
Thème du journal:
Biotechnologie
Année:
2007
Type:
Article
Pays d'affiliation:
Belgique
Institution/Pays d'affiliation:
Katholieke Universiteit Leuven/BE
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