HOX gene analysis in the osteogenic differentiation of human mesenchymal stem cells
Genet. mol. biol
;
31(4): 815-823, Sept.-Dec. 2008. tab, graf, ilus
Article
Dans Anglais
| LILACS
| ID: lil-501462
ABSTRACT
Human bone marrow-derived mesenchymal stem cells (hMSCs) have the capacity to differentiate into osteoblasts during osteogenesis. Several studies attempted to identify osteogenesis-related genes in hMSCs. Although HOX genes are known to play a pivotal role in skeletogenesis, their function in the osteogenesis of hMSCs has not yet been investigated in detail. Our aim was to characterize the expression of 37 HOX genes by multiplex RT-PCR to identify the ones most probably involved in osteogenic differentiation. The results showed that the expression patterns of four HOX genes were altered during this process. In particular, the expression levels of HOXC13 and HOXD13 were dramatically changed. Real-time PCR and Western blot analysis were performed in order to further analyze the expression of HOXC13 and HOXD13. The qRT-PCR results showed that transcription of HOXC13 was up-regulated by up to forty times, whereas that of HOXD13 was down-regulated by approximately five times after osteogenic differentiation. The Western blot results for the HOXC13 and HOXD13 proteins also corresponded well with the real-time PCR result. These findings suggest that HOXC13 and HOXD13 might be involved in the osteogenic differentiation of hMSCs.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Gènes homéotiques
/
Cellules souches mésenchymateuses
Type d'étude:
Étude pronostique
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Genet. mol. biol
Thème du journal:
Génétique
Année:
2008
Type:
Article
Pays d'affiliation:
Corée du Sud
Institution/Pays d'affiliation:
The Catholic University of Korea/KR
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