Gel mobility shift scanning of pectin-inducible promoter from Penicillium griseoroseum reveals the involvement of a CCAAT element in the expression of a polygalacturonase gene
Genet. mol. biol
;
32(1): 129-132, 2009. ilus
Article
Dans Anglais
| LILACS
| ID: lil-505768
ABSTRACT
Previous reports have described pgg2, a polygalacturonase-encoding gene of Penicillium griseoroseum, as an attractive model for transcriptional regulation studies, due to its high expression throughout several in vitro growth conditions, even in the presence of non-inducing sugars such as sucrose. A search for regulatory motifs in the 5' upstream regulatory sequence of pgg2 identified a putative CCAAT box that could justify this expression profile. This element, located 270 bp upstream of the translational start codon, was tested as binding target for regulatory proteins. Analysis of a 170 bp promoter fragment by electrophoretic mobility shift assay (EMSA) with nuclear extracts prepared from mycelia grown in pectin-containing culture medium revealed a high mobility complex that was subsequently confirmed by analyzing it with a double-stranded oligonucleotide spanning the CCAAT motif. A substitution in the core sequence for GTAGG partially abolished the formation of specific complexes, showing the involvement of the CCAAT box in the regulation of the polygalacturonase gene studied.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Penicillium
/
Polygalacturonase
/
Facteur de liaison à la séquence CCAAT
Type d'étude:
Étude pronostique
langue:
Anglais
Texte intégral:
Genet. mol. biol
Thème du journal:
Génétique
Année:
2009
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
EMBRAPA Suínos e Aves/BR
/
Universidade Federal de Viçosa/BR
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