HSV-1-derived amplicon vectors: recent technological improvements and remaining difficulties: a review
Mem. Inst. Oswaldo Cruz
;
104(3): 399-410, May 2009. ilus
Article
Dans Anglais
| LILACS
| ID: lil-517020
ABSTRACT
Amplicons are defective and non-integrative vectors derived from herpes simplex virus type 1. As the vector genome carries no virus genes, amplicons are both non-toxic for the infected cells and non-pathogenic for the inoculated organisms. In addition, the large transgenic capacity of amplicons, which allow delivery of up to 150 Kbp of foreign DNA, makes these vectors one of the most powerful, interesting and versatile gene delivery platforms. We present here recent technological developments that have significantly improved and extended the use of amplicons, both in cultured cells and in living organisms. In addition, this review also discusses the many difficulties still pending to be solved, in order to achieve stable and physiologically regulated transgene expression.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Herpèsvirus humain de type 1
/
Techniques de transfert de gènes
/
Transgènes
/
Vecteurs génétiques
Limites du sujet:
Animaux
/
Humains
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2009
Type:
Article
/
descriptif de projet
Pays d'affiliation:
France
Institution/Pays d'affiliation:
Université de Lyon/FR
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