Abundance and diversity of ammonia-oxidizing bacteria in relation to ammonium in a Chinese shallow eutrophic urban lake
Braz. j. microbiol
;
41(1): 218-226, Jan.-Mar. 2010. ilus, graf, mapas, tab
Article
Dans Anglais
| LILACS
| ID: lil-531755
ABSTRACT
The measures of most-probable-number and restriction fragment length polymorphism analysis were used to analyze the abundance and diversity of ammonia-oxidizing bacteria in sediment of a Chinese shallow eutrophic urban lake (Lake Yuehu). Among the 5 sampling sites, ammonia concentration in interstitial water was positively proportional not only to the content of organic matter, but also to ammonia-oxidizing bacteria numbers (at a magnitude of 10(5) cells g-1 dry weight) in sediment significantly. Furthermore, the diversity of ammonia-oxidizing bacteria were determined by means of PCR primers targeting the amoA gene with five gene libraries created and restriction pattern analysis. The 13 restriction patterns were recorded with 4 ones being common among all sampling sites. The 8 restriction patterns including 4 unique ones were found at the site with the highest NH4+ concentrations in interstitial water, while, there were only common patterns without unique ones at the site with the lowest NH4+ concentrations in interstitial water. Phylogenetic analysis showed that the amoA fragments retrieved belong to Nitrosomonas oligotropha & ureae lineage, N. europaea lineage, N. communis lineage and Nitrosospira lineage, most of which were affiliated with the genus Nitrosomonas. The N. oligotropha & ureae-like bacteria were the dominant species. Thus, the abundance and diversity of sediment AOB is closely linked to ammonium status in eutrophic lakes.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Techniques in vitro
/
Réaction de polymérisation en chaîne
/
Oxydants
/
Analyse de séquence d'ADN
/
Biodiversité
/
Nitrification
/
Gènes bactériens
/
Ammoniac
/
Nitrosomonas
langue:
Anglais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2010
Type:
Article
Pays d'affiliation:
Chine
Institution/Pays d'affiliation:
Institute of Hydrobiology/CN
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